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. 2017 Apr 12;6(5):1072–1081. doi: 10.1002/cam4.1053

Figure 3.

Figure 3

HIF‐1α induced up‐regulated expression of AEG‐1 and invasion capability of OVCAR3 cells. OVCAR3 cells were infected with HIF‐1αoverexpression/knockdown lentivirus for 72h. Then the cells were collected for transwell assay. Cell lysates were subjected to Western blotting analysis. The results were normalized to GAPDH to correct for loading. Data given are mean ± SD, n = 3. (A) The expression of AEG‐1 and HIF‐1α in OVCAR3 cells infected with HIF‐1α overexpression/ negative control lentivirus. **P <0.01 versus negative control. (B) The expression of AEG‐1 and HIF‐1α in OVCAR3 cells infected with HIF‐1α knockdown/negative control lentivirus. ***P <0.001 versus negative control. (C) Invasion capability of control and OVCAR3 cells after infection with HIF‐1α overexpression/knockdown and negative control lentivirus. The absorbances [optical density (OD)] of invaded cell lysates were read at 570nm. **P <0.01 versus control.