Table 2.
Basal and stimulated levels of cytokines (pg/ml) after a ti-->td:48‐hour-->48-h exposure with TNF-α (10 ng/ml) alone or TNF-α (10 ng/ml) with calcipotriol (10 nM) or dexamethasone (10 nM) or combination of all. The apparent increase of TNF-α was mostly attributable to the added TNF-α. Data comprises cell cultures from 2 to 6 patients with osteoarthritis (OA) or rheumatoid arthritis (RA). The final data is presented in Fig. 9 of the research article (the basal cytokine secretion not included) [1].
| Cytokine | Patient | no stimulation (basal) | TNF-α | TNF-α + calcipotriol | TNF-α + dex | TNF-α+ calcipotriol+ dex |
|---|---|---|---|---|---|---|
| MIP-1β | OA OA RA RA |
<13.77 <13.77 <13.77 <13.77 |
47.3 128.9 62.8 116.2 |
34.7 58.9 53.5 85.2 |
||
| IL-6 | OA OA OA OA OA OA RA RA RA RA RA RA |
349.5 301.3 860.9 686.8 |
>2971 >2971 >2971 >2971 16,350 22,863 7829 4473 5810 21,968 17,409 30,296 |
1511 2247 2643 2971 4046 3164 3859 2073 3590 9640 7455 10,031 |
802 1069 774 615 2831 1075 2692 5780 |
456 397 375 358 1420 585 1387 3568 |
| IFN-γ | OA OA OA OA OA OA RA RA RA RA RA RA |
<21.48 <21.48 <21.48 <21.48 |
41.2 56.7 52.7 38.2 57.4 61.2 67.0 51.0 66.5 88.0 54.4 78.1 |
26.5 29.4 30.5 33.8 36.5 31.3 40.1 36.0 36.4 55.8 41.6 47.1 |
24.0 30.7 27.3 29.1 40.8 33.0 36.2 57.5 |
23.3 24.0 21.8 22.1 31.3 27.6 21.9 41.5 |
| IL-1ra | OA OA RA RA |
<88.59 <88.59 <88.59 <88.59 |
147.1 159.0 148.8 201.0 |
93.1 <88.6 110.7 127.2 |
||
| IL-5 | OA OA RA RA |
<24.73 <24.73 <24.73 <24.73 |
<24.73 <24.73 <24.73 <24.73 |
<24.73 <24.73 <24.73 <24.73 |
||
| GM-CSF | OA OA OA OA OA OA RA RA RA RA RA RA |
48.19 53.72 51.83 44.88 |
81.2 77.6 56.3 73.1 70.7 75.7 79.9 88.7 83.4 92.0 63.8 59.7 |
69.3 67.2 67.4 72.1 50.6 57.1 68.5 67.8 71.2 75.4 58.1 37.3 |
66.9 68.5 66.5 61.1 70.2 68.2 72.0 76.8 |
70.8 66.0 61.1 64.1 69.5 70.0 59.7 74.5 |
| TNF-α | OA OA RA RA |
<79.83 <79.83 <79.83 <79.83 |
3160 5322 3421 3062 |
2298 2077 2533 1598 |
||
| RANTES | OA OA OA OA OA OA RA RA RA RA RA |
<24.76 <24.76 <24.76 <24.76 |
606.7 897.8 1267.0 820.6 3973.0 2887.0 302.9 1163.0 862.4 3505 2782 |
270.5 341.0 447.1 481.2 5040.0 1323.0 180.0 560.1 167.8 3080 2963 |
339.4 1267.0 660.4 1267.0 371.0 1267.0 672.7 |
133.6 363.5 268.0 363.8 160.5 442.3 167.9 |
| IL-2 | OA OA RA RA |
<13.52 <13.52 <13.52 <13.52 |
<13.52 <13.52 <13.52 15.42 |
<13.52 <13.52 <13.52 <13.52 |
||
| IL-1β | OA OA RA RA |
<30.49 <30.49 <30.49 <30.49 |
<30.49 <30.49 <30.49 <30.49 |
<30.49 <30.49 <30.49 <30.49 |
||
| Eotaxin | OA OA RA RA |
<25.51 <25.51 <25.51 <25.51 |
<25.51 <25.51 <25.51 <25.51 |
<25.51 <25.51 <25.51 <25.51 |
||
| Basic FGF | OA OA OA OA OA OA RA RA RA RA RA RA |
<11.22 <11.22 <11.22 <11.22 |
33.2 30.3 25.5 23.6 23.2 25.9 31.9 27.8 27.0 44.1 24.7 32.5 |
20.0 24.4 19.6 21.9 19.5 17.5 22.5 22.6 20.3 42.0 20.3 20.8 |
18.7 22.0 18.0 19.2 22.6 21.05 20.5 28.9 |
17.9 19.5 17.3 17.8 20.9 19.8 14.3 23.4 |
| VEGF | OA OA OA OA OA OA RA RA RA RA RA RA |
142.24 127.62 171.81 164.72 |
172.7 242.1 211.5 192.3 142.1 175.2 226.5 259.9 186.8 337.7 203.9 182.9 |
128.0 135.8 120.5 187.9 127.9 117.4 185.0 182.4 132.6 208.6 159.7 201.7 |
82.3 101.8 91.7 89.8 109.5 132.3 79.4 133.8 |
69.7 94.2 66.5 77.9 101.8 103.4 65.0 124.8 |
| PDGF-BB | OA OA RA RA |
<29.30 <29.30 <29.30 <29.30 |
<29.30 <29.30 <29.30 <29.30 |
<29.30 <29.30 <29.30 <29.30 |
||
| IP-10 | OA OA OA OA OA OA RA RA RA RA RA RA |
<33.10 <33.10 <33.10 <33.10 |
497.2 774.1 771.6 235.0 1761 789.4 275.4 1015 504.5 1727 1040 27,285 |
100.8 110.8 91.9 73.2 2238 132.7 85.0 258.9 45.8 257.2 475.1 2931 |
274.3 474.5 230.8 184.6 196.5 518.3 187.4 569.4 |
146.1 187.5 133.7 105.4 123.8 231.2 62.9 329.8 |
| IL-13 | OA OA RA RA |
<28.57 <28.57 <28.57 <28.57 |
<28.57 <28.57 <28.57 <28.57 |
<28.57 <28.57 <28.57 <28.57 |
||
| IL-4 | OA OA RA RA |
<4.52 <4.52 <4.52 <4.52 |
<4.52 <4.52 <4.52 <4.52 |
<4.52 <4.52 <4.52 <4.52 |
||
| MCP-1 | OA OA RA RA |
243.1 305.76 290.81 464.9 |
1747 2132 1052 824.7 |
2170 2559 1368 922.6 |
||
| IL-8 | OA OA RA RA |
26.44 26.73 29.68 36.96 |
11,015 15,848 11,362 9399 |
7844 8703 6805 6496 |
||
| MIP-1α | OA OA RA RA |
<16.16 <16.16 <16.16 <16.16 |
<16.16 <16.16 <16.16 <16.16 |
<16.16 <16.16 <16.16 <16.16 |
||
| IL-10 | OA OA RA RA |
<33.10 <33.10 <33.10 <33.10 |
<33.10 <33.10 <33.10 <33.10 |
<33.10 <33.10 <33.10 <33.10 |
||
| G-CSF | OA OA RA RA |
<29.78 <29.78 <29.78 <29.78 |
45.23 55.2 49.75 59.66 |
<29.78 <29.78 40.69 30.9 |
||
| IL-15 | OA OA OA OA OA OA RA RA RA RA RA RA |
<20.96 <20.96 <20.96 <20.96 |
40.6 50.9 57.2 42.3 32.4 32.4 44.8 48.3 36.4 51.6 31.4 32.9 |
25.5 33.2 38.4 22.0 30.3 28.0 30.0 39.4 13.5 44.8 30 31 |
35.0 48.9 44.6 43.9 43.0 48.9 29.4 51.1 |
29.9 36.7 34.0 29.8 35.5 40.5 2.9 44.2 |
| IL-7 | OA OA RA RA |
<32.96 <32.96 <32.96 <32.96 |
<32.96 <32.96 <32.96 <32.96 |
<32.96 <32.96 <32.96 <32.96 |
||
| IL-12(p70) | OA OA RA RA |
<15.08 <15.08 <15.08 20.06 |
<15.08 <15.08 16.25 15.98 |
<15.08 <15.08 <15.08 15.57 |
||
| IL-17α | OA OA OA OA OA OA RA RA RA RA RA RA |
<32.96 <32.96 <32.96 <32.96 |
34.2 36.1 35.9 30.3 36.8 45.1 40.9 38.0 41.1 55.0 38.3 61.2 |
<24 25.2 26.0 27.4 28.9 24.9 28.2 28.5 25.6 38.0 30.1 33.0 |
<24.2 27.1 25.2 <24.2 29.2 30.0 27.4 40.0 |
<24.2 <24.2 <24.2 <24.2 25.3 26.3 24.3 31.2 |
| IL-9 | OA OA RA RA |
<9.16 <9.16 <9.16 <9.16 |
<9.16 <9.16 <9.16 <9.16 |
<9.16 <9.16 <9.16 <9.16 |
||
| PGE2 | OA OA OA OA RA RA RA RA |
62 100 150 150 150 130 70 280 |
45 52 60 90 60 60 45 62 |
35 35 30 32 40 45 38 45 |
40 31 35 28 55 50 45 38 |