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. 2017 May 12;10(4):476–484. doi: 10.1016/j.tranon.2017.04.001

Figure 2.

Figure 2

Establishment of F2-27 and its antigens.

(A) Biotinylated Caco-2 lysate was immunoprecipitated with F2-27 or control IgG1. Proteins were detected by Western blot and silver staining. (B) EphA2 amino acid sequence. Bold letters indicate the sequence of the detected peptide. (C) Flow cytometry of the reactivity of F2-27 mAb with CHO cells transfected with EphA2 cDNA. F2-27 reacted only with CHO cells transfected with EphA2 cDNA. The numbers in each panel indicate the percentage of cells expressing EphA2.