(A) F2-27–mediated gene transduction in cancer cells.
EphA2-expressing cells (A3/KAW, MKN1, and PC-3) were treated with serum-free medium containing antibody (F2-27 or isotype IgG1 control) or no antibody (Ab−). After infection with Ax3CAZ-FZ33, β-gal activity was analyzed (n = 4). Chemiluminescent efficiencies according to β-gal expression levels were plotted at 104 VP/cell. Values are expressed as mean ± SD. *P < .01, Student's t test.
(B) Fluorescence microscopy images of the internalization and distribution of F2-27.
Immune complexes, composed of Cy5-conjugated isotype IgG1 (left) or F2-27 (right), were stained and visualized in red under a fluorescence microscope. The boxed area is shown at high magnification. Scale bars: 100 μm.