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. 2017 Mar 31;36(10):1434–1446. doi: 10.15252/embj.201695669

Figure EV1. USP49 interacts and stabilizes FKBP51.

Figure EV1

  1. SU86 cell lysates were subjected to immunoprecipitation with control IgG or anti‐FKBP51 antibody. The immunoprecipitates were then blotted with the indicated antibodies.
  2. SU86 cells stably expressing FLAG‐FKBP51 were infected with lentivirus encoding shRNAs against control or USP49, the cells were lysed, and Western blot was performed with the indicated antibodies.
  3. Quantification of the relative FKBP51 protein levels for Fig 2B. Data are represented as mean ± SEM of three independent experiments.
  4. SU86 cells stably expressing control or USP49 shRNAs were lysed and Western blot was performed with the indicated antibodies.