Skip to main content
. 2017 Mar 23;595(10):3181–3202. doi: 10.1113/JP274015

Figure 2. Purified recombinant PKI inhibits the interaction between PKA‐CS and PKA‐RS and reduces basal I Ba .

Figure 2

A, interaction of purified His‐PKA‐CS (10 μg, 0.8 μm) with GST‐PKA‐RS (RIIβ, 25 μg, 1.1 μm) was tested in the absence or presence of His‐PKI (25 μg, 7.6 μm), with and without 40 μm cAMP. His‐tagged proteins were pulled down with nickel beads, eluates were run on SDS‐PAGE gel and stained with Coomassie Blue. A representative experiment out of 4 is shown. B, PKI reduces basal I Ba. Oocytes expressing CaV1.2Δ1821 were pre‐injected with Tris‐NaCl buffer, His‐PKI, or not pre‐injected. The estimated final concentrations of PKI within the oocyte, assuming a 1 μl cell volume, are shown in μm. Maximal currents in each cell, obtained using an I–V protocol of 10 mV depolarization steps from −60 to 50 mV, were averaged and normalized to control group of the same day. Summary from 2 experiments is shown. Statistical significance was determined using one‐way ANOVA followed by Bonferroni t test.