Skip to main content
. 2017 May 15;7:94. doi: 10.3389/fonc.2017.00094

Figure 3.

Figure 3

Effects of ENMD-2076 treatment on markers of apoptosis in sensitive and resistance PDX models. (A,B) Immunofluorescence (IF) analysis of tumors harvested from CU_TNBC_002 and CU_TNBC_005 treated with vehicle control, ENMD-2076 treated at day 30 (sensitive), and ENMD-2076 treated at the time of acquired resistance. Acquired resistance was defined as a doubling in tumor volume from day 30 while receiving ENMD-2076 treatment. ENMD-2076 treatment was administered at 200 mg/kg once daily by oral gavage. (C) IF analysis of tumors harvested from CU_TNBC_004 treated with vehicle control and ENMD-2076 (intrinsically resistant to ENMD-2076). Representative IF images were acquired using confocal microscopy at 60× magnification. Scale = 5μm. Representative IHC images were taken using a Zeiss microscope at 10× magnification. Scale = 10 µm. Quantification of mean pixel intensity of the IF analysis is shown. Sensitive tumor pixel intensity was compared to vehicle control and acquired resistance pixel intensity was compared to the sensitive tumor. *p < 0.05, **p < 0.01.