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. 2017 Apr 19;7:948. doi: 10.1038/s41598-017-00935-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow virometry of HIV-1_BaL virions captured with PGT145-MNPs. (a) Virions were captured with PGT145-MNPs and stained with AlexaFluor 647-labeled PG9 (which recognizes trimeric Envs) and Pacific Blue-labeled 4B3 (which recognizes “stumps” or uncleaved Envs). Left panel: Virions were captured with PGT145-MNPs and stained with PG9 antibody. Central panel: PG9-positive PGT145-MNPs captured virions stained with 4B3. Right panel: isotype control staining. Indicated are the numbers of events. (b) Distribution of PGT145-captured virions positive for PG9 and 4B3. Each bar represents mean ± SEM of two experiments.