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. 2017 Apr 19;7:948. doi: 10.1038/s41598-017-00935-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow virometry of fluorescence-labeled HIV-1_BaL virions. (a) Alexa Fluor 488 -labeled virions were captured with PG9-MNPs (left panel) and stained with AlexaFluor 647 4B3 antibodies for the presence of stumps/uncleaved Envs (center panel) or with AlexaFluor 647-labeled isotype control antibodies (right panel). Indicated are the numbers of events. A representative experiment out of three is shown. (b) Alexa Fluor 488 -labeled virions were captured with PG16-MNPs (left panel) and stained with Pacific Blue-labeled 4B3 antibodies for the presence of stumps (center panel) or with Pacific Blue-labeled isotype control antibodies (right panel). Indicated are the numbers of events. A representative experiment out of three is shown. (c) Fractions of PG9-MNPs and PG16-MNPs captured Alexa Fluor 488 virions positive for 4B3. Each bar represents mean ± SEM of three experiments. (d) Alexa Fluor 488 -labeled virions were captured with 4B3-MNPs and stained with two antibodies: AlexaFluor 647-labeled PG9 and Pacific Blue-labeled b6. Left panel: Virions were captured with 4B3-MNPs and stained with PG9. Upper right panel: 4B3-MNP-captured virions positive for PG9 and stained with b6. Lower right panel: 4B3-MNP-captured virions negative for PG9 and stained with b6. Indicated are the numbers of events. A representative experiment out of three is shown. (e) Distribution of 4B3-MNPs–captured Alexa Fluor 488 labeled virions stained with two antibodies: AlexaFluor 647-labeled PG9 and Pacific Blue-labeled 4B3. Each bar represents mean ± SEM of three experiments.