Figure 3.

LH/CGR-promoted integrated responses in HEK293 and mLTC-1 cells. (A) HEK293 transiently co-transfected with plasmids coding for LH/CGR and pSOM-Luc reporter gene were stimulated 6 hours with increasing doses of rhCG and rhLH before luciferase activity was measured (A). mLTC-1 cells transiently transfected with plasmid coding pSOM-Luc reporter gene were stimulated 6 hours with increasing doses of rhCG and rhLH before luciferase activity was measured (B). For LHR-mediated steroid production, mLTC-1 cells were stimulated 3 hours with increasing doses of rhCG and rhLH and the supernatant levels of progesterone (C) and testosterone (D) were measured by ELISA or HTRF, respectively. Data are means ± SEM of 3–5 independent experiments performed in single or duplicate.