Table 2.
Efficacy (E max) and efficiency (EC 50) of rhCG and rhLH on LHR measured in different assays in mLTC-1 cells.
| Responses | rhCG | rhLH | ||||
|---|---|---|---|---|---|---|
| E max | EC 50 | n | E max | EC 50 | n | |
| cAMP (BRET) | 0.45 ± 0.02 | 68.82 ± 22.30 pM | 5 | 0.44 ± 0.02ns | 459 ± 105.35 pM** | 5 |
| cAMP (HTRF) | 100% | 97.37 ± 47.15 pM | 3 | 102 ± 5.8%ns | 1980.33 ± 868.64 pM* | 3 |
| Cre-reporter assay | 1910 ± 111 | 24.97 ± 16.20 pM | 4 | 1971 ± 168ns | 317.62 ± pM* | 4 |
| Progesterone | 100% | 19.72 ± 4.28 pM | 3 | 49.78 ± 2.90%*** | 305.83 ± 25.22 pM** | 3 |
| Testosterone | 100% | 2.19 ± 0.91 pM | 4 | 93.33 ± 6.5%ns | 29.05 ± 6.90 pM** | 4 |
The Emax values are represented as hormone-induced BRET changes (for cAMP by BRET), % of rhCG-induced response (for cAMP by HTRF, progesterone and testosterone) and relative luciferase activity (for Cre-reporter assay). Statistical analyses were performed with unpaired t-test (***p < 0.001; **p < 0.01; *p < 0.05, ns non-significant).