Figure 5.

AAV co-transduction of sensory neurons in the trigeminal ganglia. (a,b) Swiss Webster mice were inoculated by intradermal injection of the whiskerpad with PBS or with 2 × 10¹¹ vector genomes of scAAV1-smCBA-mCherry and 2 × 10¹¹ vector genomes of scAAV1-smCBA-GFP. At 21 days post AAV delivery trigeminal ganglia were harvested and primary explant cultures were established to analyze numbers of mCherry and GFP positive cells by fluorescence microscopy. Representative images of primary sensory neuronal cultures are shown for 3 (a) and 29 days (b) post explant. For quantification GFP and mCherry positive neurons were counted in a total of 10 fields of view for each neuronal culture over 29 days.