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. 2017 Apr 19;7:924. doi: 10.1038/s41598-017-01069-9

Figure 4.

Figure 4

Notch1 pathway upregulates TGF-β1/Smad3 pathway. (A) We cultured A549 cells for 6 days, with PQ (300 μmol/L), PQ + Jagged-1(500 ng/mL), or PQ + DAPT (10 μmol/L). The mRNA level of TGF-β1 and Smad3 was increased after induction by Jagged-1, while DAPT could inhibit the mRNA of TGF-β1 and Smad3 byqRT-PCR. (B) A549 cells were cultured with PQ or PQ + Jagged-1, incubated in the presence or absence of SB431542 (a specific inhibitor of TGF-β receptor type I/ALK5 kinase that phosphorylates Smad2/3, 10 μmol/L) for 6 days. Jagged-1 significantly induced α-SMA, and this induction was completely inhibited by SB431542 in A549 cells by western blot analysis. (C) Cell membrane distribution of α-SMAand E-cadherin protein in A549 cells. Jagged-1 significantly induced expression of α-SMA protein, and this induction was inhibited by SB431542 in A549 cells. α-SMA was stained green, E-cadherin was stained red and DAPI stained nuclei blue. *p < 0.05.