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. 2017 Apr 19;7:943. doi: 10.1038/s41598-017-01176-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of AHL-degradation activity. (a) TLC analysis using the biosensor Agrobacterium tumefaciens NTL4 (pZLR4). (b) HPLC/MS measurements of remaining C₆-HSL and C₁₂-HSL after 24 h incubation with E. coli S17 λ pir and E. coli S17 λ pir::f10/17.1H. LB medium was used as control. Initial AHL concentration was 25 µM. Error bars represent standard deviations. Different letters above the bars indicate that the values are significantly different according to a one-factor (treatment) ANOVA (P < 0.05) and the Tukey test.