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. 2017 Apr 19;7:915. doi: 10.1038/s41598-017-01003-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of miR-143 and miR-145 in the eye as revealed by qRT-PCR and LacZ staining. LacZ plus eosin counter-staining was performed in ~2-month old transgenic mice in which LacZ is driven by a 5 kb miR-143/145 enhancer. (A) Relative expression of miR-143 in HTM cells compared to HUVEC and HASMC cells. **p < 0.01; (B) Relative expression of miR-145 in HTM cells compared to HUVEC and HASMC cells. ***p < 0.001; (C) Relative expression of miR-143/145 in HTM cells compared to HCEC and ARPE-19 cells. ***p < 0.001; (D) LacZ staining was observed in smooth muscle (a), pericytes (b), ciliary muscle (c), extraocular muscle (d,e), but not in the corneal epithelial layer (f) in the eye.