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. 2017 Apr 19;7:964. doi: 10.1038/s41598-017-01029-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative real-time PCR (qRT-PCR) validations of 26 differentially expressed genes in AC (red), AM (green) and AF (blue). For each qRT-PCR validation, three technical replications were performed, beta-tubulin gene was used as internal control. All results were expressed as means ± standard error (SE) of the number of experiments. The lowercase ‘a’, ‘b’ and ‘c’ indicated that statistically significant difference of mRNA level was considered on an error probability of p < 0.05 by one-way ANOVA using Duncan’s multiple-range test.