Table 1.
Effect of sgRNA and Cas9 protein concentration on N. vitripennis survival and mutagenesis.
| sgRNA-1 | Cas9 | Total embryos | Adult Survivors | Mosaic (%) | ||||
|---|---|---|---|---|---|---|---|---|
| ♂ | ♀ | Total (%) | M (%) | F (%) | Both (%) | |||
| No injection | No injection | 100 | 66 | 26 | 92 (92) | 0 (0) | 0 (0) | 0 (0) |
| Water | Water | 100 | 44 | 32 | 76 (76) | 0 (0) | 0 (0) | 0 (0) |
| 20 (ng/ul) | 20 (ng/ul) | 100 | 34 | 34 | 68 (68) | 0 (0) | 0 (0) | 0 (0) |
| 40 | 40 | 100 | 30 | 32 | 62 (62) | 4 (13) | 0 (0) | 4 (6) |
| 80 | 80 | 100 | 24 | 22 | 46 (46) | 6 (25) | 0 (0) | 6 (13) |
| 160 | 160 | 100 | 16 | 22 | 38 (38) | 5 (31) | 3 (14) | 8 (21) |
| 320 | 320 | 100 | 10 | 10 | 20 (20) | 6 (60) | 6 (60) | 12 (60) |