Table 3.
Molecular quantification of SbGP/MPV phytoplasma in infected plant samples obtained in 2014.
| Sample | Method Unit | qRT-PCR genomes/g tissue | ddPCR genomes/g tissue | LAMP Time to positive (Tp), minutes (calcein detection) | |||
|---|---|---|---|---|---|---|---|
| Tissue | Mean (n) | Standard deviation | Mean (n) | Standard deviation | Mean (n) | Standard deviation | |
| S31b-L-MA | Leaf midrib | 4.82 × 108 (10) | 5.48 × 107 | 2.41 × 108 (10) | 2.51 × 107 | 45.88 (2) | 3.01 |
| S31b-GP-MA | Green petal | 2.74 × 109 (2) | 7.58 × 107 | 4.80 × 108 (2) | 4.88 × 107 | 31.88 (2) | 3.00 |
| S31b-P-MA | Fruit peduncle | 4.34 × 108 (2) | 2.01 × 107 | 2.11 × 108 (2) | 2.61 × 107 | 35.0 (3) | 0.43 |
| S31b-F-MA | Fruit pulp | 9.69 × 107 (2) | 1.80 × 107 | 5.48 × 107 (2) | 4.24 × 106 | 45 (2) | 3.54 |
| S289-L-MA | Leaf midrib | 1.34 × 108 (10) | 1.78 × 107 | 7.94 × 107 (9) | 1.14 × 107 | 49.63 (2) | 3.01 |
| S289-GP-MA | Green petal | NDa (2) | ND (2) | ND (2) | |||
| S289-P-MA | Fruit peduncle | 4.24 × 108 (2) | 1.29 × 107 | 7.16 × 107 (2) | 3.61 × 106 | 35.38 (2) | 0.18 |
| S289-F-MA | Fruit pulp | ND (2) | ND (2) | ND (2) | |||
| S26b-GP-MA | Green petal | 1.12 × 109 (2) | 7.44 × 107 | 3.85 × 108 (2) | 7.64 × 106 | 35.38 (2) | 1.24 |
| S26b-P-MA | Fruit peduncle | 1.04 × 109 (2) | 2.90 × 108 | 4.23 × 108 (2) | 6.36 × 106 | 25.25 (2) | 0 |
| S26b-F-MA | Fruit pulp | 3.98 × 108 (2) | 1.22 × 107 | 1.46 × 108 (2) | 1.09 × 107 | 38.38 (2) | 0.18 |
| S27b-GP-MA | Green petal | 4.01 × 109 (2) | 4.07 × 108 | 6.92 × 108 (2) | 1.23 × 108 | 30.88 (2) | 1.59 |
| S267-GP-MA | Green petal | 8.21 × 102 (4) | 4.53 × 102 | ND (2) | ND (2) | ||
| S267-P-MA | Fruit peduncle | ND (2) | ND (2) | ND (2) | |||
| S267-F-MA | Fruit pulp | ND (2) | ND (2) | ND (2) | |||
| P83-L-SLP | Leaf midrib | 4.67 × 109 (10) | 7.72 × 108 | 2.53 × 109 (10) | 1.80 × 108 | 38.42 (3) | 2.31 |
| P86-L-SLP | Leaf midrib | 6.13 × 109 (10) | 8.46 × 108 | 2.82 × 109 (10) | 3.10 × 108 | 37.08 (3) | 2.13 |
| Strawberry (H)b | Leaf | ND (2) | ND (2) | ND (2) | |||
| Periwinkle (H) | Leaf | ND (2) | ND (2) | ND (2) | |||
| Raspberry (H) | Fruit | ND (2) | ND (2) | ND (2) | |||
| Blueberry (H) | Leaf | ND (2) | ND (2) | ND (2) | |||
| Blackberry (H) | Fruit | ND (2) | ND (2) | ND (2) | |||
| Nontargetc | ND (2) | ND (2) | ND (2) | ||||
aND, not detected. bH, DNA from uninfected plant. See Supplementary Fig. S4 for results. cNontarget DNA is a mixture of plasmids consisting of 106 copies each of all of the cpn60 UT fragments from the phytoplasma strains described in Dumonceaux et al.12. See Supplementary Fig. S4 for results on individual templates.