Figure 10.

Optic Nerve Morphology in Retinas Transduced with AAV/2-NGB

(A) Proximal ON transverse sections were subjected to immunohistochemical staining with antibodies against NF200 (green) and GFAP (red). Results obtained from four mice are shown. From top to bottom: untreated 2-month-old mouse; untreated 1-year-old mouse; 1-year-old mouse subjected to early treatment; and 1-year-old mouse subjected to late treatment. The scale bar represents 50 μm. (B) Bar chart of estimated axon numbers (NF200-positive spots) for mice in the same groups as in (A); the number of mice evaluated in each group is shown in brackets below each bar (n). p values were calculated with respect to the number of fibers in untreated 2-month-old DBA/2J mice. (C) Results of immunohistochemical staining with antibodies against IBA1 (green) and vimentin (red) of ON sections from three 12-month-old mice. From top to bottom: untreated mouse; mouse treated at 2 months of age (early treatment); and mouse treated at 8 months of age (late treatment). The nuclei were stained with DAPI (blue). The scale bar represents 50 μm. (D) Normalized values (against the mean fluorescence in 12-month-old mice) for GFAP, IBA1, and vimentin labeling for the number of ON sections indicated beneath each bar (n). The values were plotted using GraphPad Prism 6 as means ± SEMs.