Figure 8.

Morphology of Neurons in the Ganglion Cell Layer after Early Treatment with AAV2/2-NGB

Flat-mounted retina preparations (vitreal side up, with the GCL visible) were subjected to immunohistochemical staining with antibodies against BRN3A (red) and β3-tubulin (green). The results shown were obtained with three retinas: one isolated from a 2-month-old untreated mouse and two from a 12-month-old mouse subjected to AAV2/2-NGB administration at the age of 2 months (early treatment) and euthanized 10 months later. The observations were performed with a confocal microscope and regions from the periphery are illustrated. A composite image for BRN3A and β3-tubulin labeling is shown at the right. The nuclei were stained with DAPI (blue). The scale bars represent 25 μm. In the β3-tubulin panel, some BRN3A-positive cells are highlighted: white arrowheads indicate cells displaying short or few dendrites (untreated retina), and red arrowheads indicate cells with long dendrites (transduced retinas).