Figure 9.

Morphology of Neurons in the Ganglion Cell Layer after Late Treatment with AAV2/2-NGB

Flat-mounted retinas from two untreated 12-month-old mice were compared to retinas from two mice that underwent intravitreal injection of AAV2/2-NGB in each of their eyes at 8 months of age (late treatment). The confocal images shown correspond to the peripheral region of the retinas. A composite image for BRN3A and β3-tubulin labeling is shown at the right. The nuclei were stained with DAPI (blue). The scale bars represent 25 μm. In the β3-tubulin panel, some BRN3A-positive cells are highlighted: white arrowheads indicate cells displaying short dendrites (untreated retinas from old mice), and red arrowheads indicate cells with long dendrites (transduced retinas).