Table 1.
Respiratory Chain Complex Enzymatic Activities in Retinas of DBA/2J Mice
| Specific Activity ± SEM |
|||||
|---|---|---|---|---|---|
| 2 Months | 5 Months | 8 Months | 10 Months | 14–16 Months | |
| Complex Ia | 40.5 ± 1.9 (n = 26)b | 32.14 ± 1.7 (n = 10) | 31.8 ± 1.1 (n = 10) | 21.5 ± 1.6 (n = 18) | 24.7 ± 1.6 (n = 20) |
| p value versus 2-month-old mice | 0.02 | 0.009 | <0.0001 | <0.0001 | |
| Complex V | 103 ± 4.2 (n = 26) | 96.7 ± 7.7 (n = 10) | 102.8 ± 5.0 (n = 10) | 94.8 ± 4.3 (n = 18) | 97.1 ± 6.6 (n = 20) |
| p value versus 2-month-old mice | 0.41 | 0.99 | 0.26 | 0.85 | |
| CI/CV | 0.42 ± 0.03 | 0.36 ± 0.03 | 0.30 ± 0.02 | 0.24 ± 0.021 | 0.25 ± 0.016 |
| p value versus 2-month-old mice | 0.37 | 0.02 | <0.0001 | <0.0001 | |
| Complexes II + IIIc | 48.5 ± 4.2 (n = 24) | ND | ND | 30.3 ± 2.6 (n = 17) | 24.3 ± 1.6 (n = 22) |
| p value versus 2-month-old mice | 0.0055 | <0.0001 | |||
| Complex IIId | 247.9 ± 31.3 (n = 24) | ND | ND | 86.1 ± 9.5 (n = 17) | 82.9 ± 12.1 (n = 22) |
| p value versus 2-month-old mice | 0.0007 | <0.0001 | |||
| Complex IVe | 357.0 ± 9.7 (n = 24) | ND | ND | 255.3 ± 19.4 (n = 17) | 246.4 ± 13.97 (n = 22) |
| p value versus 2-month-old mice | <0.0001 | <0.0001 | |||
a
Complex I and complex V activities are expressed as nanomoles of oxidized “NADH/min/mg protein”.
b
The number of independent retinas evaluated is indicated in brackets.
c
Complex II + III activity is expressed as nanomoles of reduced “cytochrome c/min/mg protein”.
d
Complex III activity is expressed as nanomoles of “oxidized decylubiquinone/min/mg protein”.
e
Complex IV activity is expressed as nanomoles of “oxidized cytochrome c/min/mg protein”.