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. 2017 Apr 20;7:978. doi: 10.1038/s41598-017-00981-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup. Experimental setup consisting of a HD-MEA system, a patch clamp system, and an upright microscope. The HD-MEA is controlled through an FPGA and a Linux computer. A windows computer controls the patch-clamp amplifier settings and the microscope. The microscope is mounted on an XY-stage, which allows for determining the exact positioning for every acquired image. The Linux workstation is used to record the combined intra- and extracellular data, to visualize the data, to align acquired images to the MEA coordinates, and to run the experimental protocols.