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. 2017 Apr 20;7:993. doi: 10.1038/s41598-017-01102-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ab-initio modelling of CBD12 from NCX2 and NCX3. (A,D,G) Experimental SAXS curves of CBD12 from NCX2 (A), NCX3-AC (D), and NCX3-B (G) in the presence (red) and absence (black) of Ca²⁺. (B,E,H) Paired-distance distribution functions of CBD12 from NCX2 (B), NCX3-AC (E), and NCX3-B (H) in the presence (red) and absence (black) of Ca²⁺ as determined using GNOM. (C,F,I) Ab-initio model of CBD12 from NCX2 (C), NCX3-C (F), and NCX3-B (I) in solution in the presence of Ca²⁺. The homology model of CBD12 was fit into the molecular envelope using SUPCOMB. CBD1 and CBD2 are presented as orange and red cartoons, respectively.