Table 1.

.

Sample	NCX2-CBD12		NCX2-CBD12-AC		NCX2-CBD12-B
Ligand	10 mM Ca2+	10 mM EDTA	10 mM Ca2+	10 mM EDTA	10 mM Ca2+	10 mM EDTA
Data collection parameters
Beamline	ESRF BM29
Beam geometry (mm2)	0.5 × 0.5
Wavelength (Å)	1.0
Q range (Å−1)	0.0025–0.5
Exposure time per frame (seconds)1	1
Concentration range (mg/ml)	1.5–10		1.5–11.5		1.5–11.25
Temperature (°C)	5
Structural parameters
Rg (Å) [from P(r)]2	34.9 ± 0.01	32.5 ± 0.007	33.9 ± 0.006	35.0 ± 0.01	34.1 ± 0.007	33.5 ± 0.01
Rg (Å) (from Guinier)2	32.6 ± 0.1	31.5 ± 0.2	32 ± 0.1	36.2 ± 0.4	32.9 ± 0.1	33.5 ± 0.3
Dmax (Å)3	120 ± 12	108 ± 11	115 ± 12	120 ± 12	115 ± 12	120 ± 12
Porod volume [from P(r)] (103 Å3)	45.9	70.0	39.5	72.5	40.5	56.3
NSD4	0.85 ± 0.04	NA	0.81 ± 0.05	NA	0.81 ± 0.05	NA
χ2 (EOM)	1.5	NA	0.65	NA	0.66	NA
Software employed
Primary data reduction	AUTOMAR
Data processing	PRIMUS
Modelling	DAMMIF, EOM

¹Ten frames were measured for each sample. ²±S.E. ³±10% (estimated range). ⁴NSD, normalized shape discrepancy for DAMMIF calculation.