Skip to main content
. 2017 Apr 20;7:979. doi: 10.1038/s41598-017-00888-0

Figure 2.

Figure 2

The effect of obestatin on GSIS is mediated by GHS-R in β-cells. (A) INS-1 cells were treated with 5 µM GHS-R antagonists YIL781 or JMV2959 for 1 hour, and insulin secretion was measured at 22.2 mM glucose with or without 100 nM obestatin. #P < 0.05, treatment vs control; *P < 0.05 obestatin vs obestatin with GHS-R antagonist, n = 8–14. (B) GHS-R was transiently knocked down in INS-1 cells and gene expression was measured after 48 hours. (C) Scrambled or siGhsr transfected INS-1 cells were treated with different doses of obestatin at 22.2 mM glucose for 60 minutes. Insulin was normalized to total protein content of cells. #P < 0.05 obestatin treatment vs control for each genotype, *P < 0.05 scrambled vs siGhsr, n = 5.