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. 2017 Apr 20;7:981. doi: 10.1038/s41598-017-01056-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of TTX (1 µM) on cardiomyocyte contraction function and intracellular calcium transient in the presence of Bay K 8644 (300 nM). (a) and (b) Representative recordings of myocardial sarcomere length (a) and intracellular calcium transients (b) under different conditions. (c) The myocardial contraction amplitude (n = 25), and calcium transient amplitude (n = 16) in control, Bay K 8644, and Bay K 8644 plus TTX. (d) The intracellular calcium concentration ([Ca²⁺]_i) in myocardial cells in control, Bay K 8644, and Bay K 8644 plus TTX, n = 16. ^* P < 0.05 vs. control; ^# P < 0.05 vs. Bay K 8644.