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. 2017 Apr 20;7:936. doi: 10.1038/s41598-017-01039-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Percent mortality at 48 hours for male and female locusts after injection with imidacloprid (IMD) solutions ranging in concentrations from 10 to 10,000 ng/g (plotted on log scale), fitted with iterative non-linear regressions (solid lines). Points represent proportions from groups of 6 to 12 locusts, normalized to the mortality of vehicle control groups used on each testing day. Regression lines revealed an LD50 of approximately 2,500 ng/g for males. The LD50 for females was beyond the highest concentration used.