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. 2017 Apr 20;7:970. doi: 10.1038/s41598-017-01072-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protein expression of specific markers in HKs within hybrid scaffolds. Images (left) show the fluorescent staining of ALDH3A1 (green) and nuclei (blue) in HKs after being cultured for 1, 3, and 7 days. Protein expression of specific markers in HCECs seeded on the surface of hybrid scaffolds. Images (right) show fluorescent staining of CK3 (green) and nuclei (red) in HCECs after being cultured for 1, 3, and 7 days. (scale bar, 200 μm).