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. 2017 Apr 20;7:971. doi: 10.1038/s41598-017-01065-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment of donor corneas with IL-10 and TGFβ1 decreases infiltration of immune cells in the grafts. Two days after single subconjunctival injection of IL-10 and TGFβ1 or saline, corneas were transplanted on high-risk graft beds. Infiltration of leukocytes into the graft was determined 2 weeks after transplantation using flow cytometry. Corneal grafts were analyzed for frequencies of (A) MHCII⁺CD45⁺ cells and (B) CD4⁺ T cells. Bar graphs demonstrate mean ± SEM; N = 5 mice/group, data shown are representative of two independent experiments.