Table 1.
Influence of abscisic acid (ABA) on induction of somatic embryos in callus of P. japonicum
| ABA (mg/L) | Percentage of callus clumps showing somatic embryo | Total number of somatic embryos | Average callus fresh weight/clump (mg)a |
|---|---|---|---|
| 0 | 15 | 10 (t) | 447 ± 48 a |
| 0.5 | 15 | 27 (g) | 336 ± 16 b |
| 1.0 | 20 | 44 (g) | 329 ± 42 b |
| 2.0 | 25 | 17 (g) | 212 ± 27 c |
| 4.0 | 30 | 13 (g) | 172 ± 12 d |
Culture medium: MS basal medium + 3 % sucrose + 0.4 % GPP. Total number of callus clumps = 20 (10 in each petridish); each callus clump = 100 mg; Observations at 60 days of culture
g globular, t torpedo
aMean ± standard error. Means within each column followed by the same letter(s) are not significantly different at 5 % level by Fisher's protected LSD test