Table 2.
Influence of different light spectra on induction of somatic embryos and callus growth in P. japonicum
| Light spectrum | Total number of somatic embryos | Percentage of vitrified somatic embryos | Average callus fresh weight (mg/clump)a | Callus texture |
|---|---|---|---|---|
| CW-5000 K | 110 (g) | 20 | 732 ± 37 b | Compact, dry, pink color |
| WW-2700 K | 90 (g + t) | 31 | 644 ± 14 de | Compact, succulent |
| 7R1G1B | 191 (g + t) | 20 | 635 ± 18 def | Compact, succulent |
| 8R1B | 329 (g + t) | 20 | 555 ± 14 ef | Compact, light golden color |
| 3R3B3IR | 0 | 0 | 721 ± 16 bc | Compact, dry, with white cells |
| 9IR | 0 | 0 | 821 ± 15 a | Compact, deep golden color |
| 9R | 0 | 0 | 631 ± 6 def | Compact, light creamish color, succulent |
| 9B | 0 | 0 | 681 ± 11 bcd | New callus friable, purple color, succulent |
Culture medium: MS basal medium + 3 % sucrose + 0.4 % GPP. Total number of callus clumps each petridish = 20; each callus clump = 100 mg; observations at 60 days of culture
g globular, t torpedo
aMean ± standard error. Means within each column followed by the same letter(s) are not significantly different at 5 % level by Fisher's protected LSD test