Fig. 1.

Cell-surface PDI is not externalized by the ER-Golgi pathway in VSMC. (A) Primary VSMC were treated for 4.5 h with BFA 18 µM or Mon 18 µM and underwent cell-surface biotinylation, followed by staining with anti-PDI RL90. All data are mean±SEM from three separate experiments. (B) Immunofluorescence staining of PDI (in red) in non-permeabilized VSMC in basal condition or after treatment with BFA 10 µM or Mon 10 µM for 4.5 h. All immunofluorescence data were obtained with anti-PDI SPA891, which recognizes the C-terminal domain. All images are representative of at least three independent experiments. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)