Fig. 3.

Western blot and detection of the GFP fluorescence signal in transgenic orchids. a Western blot analysis of the expression of GFP protein in transgenic orchids. Total protein was isolated from the leaf of orchid and 30 μg total protein was loaded into 10 % acrylamide gel, electrophoresed, and transferred onto polyvinylidene fluoride (PVDF) membrane for antibody probing. The membrane was probed using an anti-GFP antibody and anti-actin antibody served as the loading control. Wt wild-type; 4n tetraploid; 2n diploid. b GFP fluorescence signal was detected in D20 transgenic Phalaenopsis aphrodite. c A magnified view of the GFP signal in partial tissue of b. Root tissues of the D20 transgenic line and Wt were vertical and transversely sectioned, and leaves were transverse-sectioned using a vibratome and an image was obtained using a confocal microscope