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. 2017 Apr 21;7:1041. doi: 10.1038/s41598-017-01015-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phasor analysis of NAD(P)H fluorescence lifetime. (a–d) Phasor plots of neurons and astrocytes with and without Mn plotted along a standard curve intersecting 5.5 ns and 0.31 ns. (a,b) Neurons treated with Mn (b) have a phasor distribution shifted towards bound NAD(P)H relative to the control (a). (c,d) Astrocytes treated with Mn (d) have a phasor distribution shifted towards free NAD(P)H relative to the control (c). (e–h) These shifts are echoed in bound fraction maps formed from a representative image from each group.