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. 2017 Apr 25;7:1131. doi: 10.1038/s41598-017-01258-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphorylation of serine residues juxtaposed to LIR of Nix enhances interaction of Nix to LC3 protein family. (a) Alignment of LIR motif of Nix, Bnip3 and Optineurin. (b) GST pull-down of GST-LC3A and GST-LC3B and Nix wt and Nix W36A, double Nix S34,35A, Nix S34,35D, Nix S34,35E mutants and single Nix S34A, Nix S34E, Nix S35A, Nix S35E mutants. TCL corresponds to total cell lysate.