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. 2017 Apr 25;7:1131. doi: 10.1038/s41598-017-01258-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LC3B Arg10, Arg11 and Lys49 residues are important for interaction with phosphorylated Nix. (a) GST pull-downs with GST-LC3B wild type GST-LC3B, R10A, R11A, K49A and K51A were performed against Flag-Nix wild type, S34,35A and S34,35E. (b) Cells cotransfected with Nix wild type, ΔLIR, S34,35A, S34,35E or S34,35D and GFP-LC3B were screened using immunofluorescent microscopy. Three independent experiments were performed and average numbers of LC3B positive vesicles per 100 cells in each experiment were calculated. Error bars are standard deviation. *Significantly different from the control Nix wild type, p < 0.05.