Table 1.

Thermodynamic parameters of interaction between LC3B (wild type and mutants), and Nix and p62 LIRs.

	ΔH kcal mol−1	ΔS cal mol−1 K−1	−T*ΔS kcal mol−1	ΔG kcal mol−1	Ka *106 M−1	Kd μM	N
Nix LIR P0
WT*	−2.72 ± 0.07	+9.4	−2.80	−5.52	0.011 ± 0.01	91	1**
WT	−2.58 ± 0.13	+9.7	−2.88	−5.46	0.010 ± 0.001	99	1**
K49A	−5.41 ± 0.50	+4.7	−1.40	−6.81	0.097 ± 0.009	10.3	0.98 ± 0.08
Nix LIR P2
WT*	−6.57 ± 0.04	+5.7	−1.71	−8.27	1.17 ± 0.05	0.85	1.06 ± 0.01
WT	−4.40 ± 0.01	+13.1	−3.90	−8.30	1.21 ± 0.06	0.83	1.08 ± 0.01
R11A	−3.00 ± 0.01	+16.3	−4.86	−7.86	0.593 ± 0.05	1.69	1.06 ± 0.01
K49A	−5.44 ± 0.01	+9.9	−2.96	−8.40	1.43 ± 0.07	0.70	1.02 ± 0.01
K51A	+0.74 ± 0.01	+23.4	−6.97	−6.23	0.039 ± 0.01	26	1**
p62
WT*	−10.5 ± 0.13	−8.7	+2.59	−7.97	0.68 ± 0.04	1.47	0.99 ± 0.01
WT	−9.38 ± 0.17	−5.4	+1.60	−7.78	0.498 ± 0.03	2.01	1.04 ± 0.01
R11A	−8.22 ± 0.23	−3.0	+0.90	−7.32	0.233 ± 0.05	4.29	1.06 ± 0.02
K49A	−10.84 ± 0.01	−8.9	+2.65	−8.19	1.02 ± 0.05	0.98	0.99 ± 0.01
K51A	−4.70 ± 0.01	+5.0	−1.48	−6.18	0.034 ± 0.01	29	1**

*Previous set of conditions reported by Novak et al.¹⁶ −50 mM NaPi, 150 mM NaCl, pH = 7.5.

**N was fixed to 1.00 upon fitting.