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. 2017 Apr 21;7:1020. doi: 10.1038/s41598-017-01093-9

Figure 5.

Figure 5

PTC124 has an off-target effect on PLuc and is not restoring mLEPR-bW31X signalling. (a) Lysates from HEK293 cells containing PLuc and RLuc were treated with rising concentrations of PTC124 during bioluminescence quantification. Shown is one representative measurement. (b) HEK293 cells were co-transfected with the STAT3-RE-PLuc and STAT3-RE-Rluc vector. Two days after the transfection, cells were treated for 24 h with rising concentrations of PTC124 (n = 3, SD). (b) HEK293 cells were co-transfected with the mLepr-b wt overexpression construct and STAT3-RE-RLuc. Two days after the transfection, cells were treated for 16 h with rising concentrations of murine leptin. Shown is one representative measurement. (d,e) HEK293 cells were co-transfected with either the mLepr-b wt- or mLepr-b W31X-pcDNA3.1 overexpression construct and with STAT3-RE-RLuc. Two days after the transfection, cells were treated for 24 h with G418 or with rising concentrations of PTC124 and for the last 16 h in addition with 6 nM murine leptin (n = 3, SD). Statistical significance between (−) and (+) leptin was assessed by t-tests with Bonferroni-Holm correction for multiple comparisons ***p < 0.001.