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G418 but not PTC124 suppresses nonsense mutations in the secreted NanoLuc luciferase. HEK293 cells were transfected with either the secNLuc W40X and secNLuc W162X overexpression construct. Two days after the transfection, cells were treated for 24 h, 48 h and 72 h with rising concentrations of G418 (a–c) or PTC124 (d–f) (n = 4, SD). Luciferase activities were measured either in medium or in cell lysates.
