Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 28;7:1277. doi: 10.1038/s41598-017-01215-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Changes in human astrocytes’ secreted and intracellular amounts of endogenous Aβ₄₂ and Aβ₄₀. (a) Aβ₄₂ peptides secreted amounts increase after an exposure to fAβ₂₅ ₋₃₅ ± CMT, but stay unchanged when NPS 2143 is also added. Points on the curves are means ± SEM of 3–5 independent experiments; fAβ_25–35 + CMT ± NPS 2143: one-way ANOVA analysis of the complete data set F = 140.805, P < 0.001. Bonferroni t-test: comparisons of each treated group versus 0-h group (controls): *P < 0.05; pair-wise comparisons between corresponding treatments ± NPS 2143 at each time point: #P < 0.05. (b) Changes in Aβ₄₀ released amounts according to the treatments. Points on the curves are means ± SEM of 3–5 independent experiments; fAβ_25–35 + CMT ± NPS2143: one-way ANOVA analysis of the complete data set F = 13.871, P < 0.001. Bonferroni t-test: comparisons of each treated group versus 0-h group (controls): *P < 0.05. (c) Left panel. Typical immunoblot revealing the changes in intracellularly accumulated Aβ₄₂/Aβ₄₂-os versus untreated controls according to treatments. LC, loading control. Right panel. Densitometric evaluations of the whole sets of intracellular Aβ₄₂ bands at each time point. Points on the curves are means ± SEM of 3 independent experiments, with control values normalized as 1.0. One-way ANOVA analysis of the complete data set F = 3.933, P < 0.012. Bonferroni t-test: comparisons of each treated group versus 0-h group (controls): *P < 0.05. (d) Alterations in total 0-to-72-h amounts of secreted Aβ₄₂ according to treatments. Bars are means ± SEM of 5 independent experiments. One-way ANOVA analysis of the complete data set F = 13.248, P < 0.001. Bonferroni t-test: comparisons of each treated group versus 0-h group (controls): *P < 0.05; pair-wise comparisons between corresponding treatments ± NPS 2143: #P < 0.05. (e) Changes in secreted Aβ₄₂/Aβ₄₀ ratio values according to treatments in the conditioned growth media of astrocytes. Bars are means ± SEM of 3 independent experiments. One-way ANOVA analysis of the complete data set F = 73.769, P < 0.001; Bonferroni t-test: comparisons of each treated group versus 0-h group (controls): *P < 0.05; pair-wise comparisons between corresponding treatments ± NPS 2143 at each time point: #P < 0.05.