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. 2017 Apr 21;7:1053. doi: 10.1038/s41598-017-01165-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-PCR validation of the splice junctions solely detected by one algorithm using bulk mRNA. Comparison of the splice junctions detected by FANSe2splice versus TopHat2, HISAT2, MapSplice2 and STAR, for HBE and A549 cell lines, respectively. The junctions solely detected by one algorithm were tested by RT-PCR. Small pie charts denote the fraction of validated known junctions (yellow) and novel junctions (cyan). The junctions identical to other known junctions were marked as black. Complete experimental evidences for all RT-PCR validations are listed in Supplementary Figs S3–S6. Please see main text for more details.