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. 2017 Apr 27;7:1237. doi: 10.1038/s41598-017-01407-x

Figure 3.

Figure 3

Heterologous expression of CsHSP17.2 in E. coli. (A) Expression of the CsHSP17.2 protein in E. coli assessed by SDS-PAGE analysis. M, protein molecular weight marker; lane 1: whole cell lysate of un-induced cells containing the empty vector pET; lane 2: whole cell lysate of cells cntaining the empty vector and induced by 0.2 mM IPTG at 15 °C for 16 h; lane 3: whole cell lysate of cells containing the empty vector and induced by 0.2 mM IPTG at 37 °C for 4 h; lane 4: whole cell lysate of un-induced cells containing pET-HSP17.2; lane 5: whole cell lysate of cells containing pET-HSP17.2 and induced by 0.2 mM IPTG at 15 °C for 16 h; lane 6: whole cell lysate of cells containing pET-HSP17.2 and induced by 0.2 mM IPTG at 37 °C for 4 h. The induced proteins are indicated by black arrows. (B) Growth analysis of transformed E. coli BL21 (DE3) cells under normal conditions. (C) Protective effect of recombinant CsHSP17.2 on cell viability during HS in vivo. The mean values of three independent experiments are plotted with error bars indicating standard deviations. The significant differences are indicated by different letters (P < 0.05).