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. 2017 Apr 27;7:1237. doi: 10.1038/s41598-017-01407-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thermotolerance of WT and transgenic A. thaliana. (A) RT-PCR identification of CsHSP17.2 transgenic A. thaliana lines. Total RNA was extracted from rosette leaves of 4-week-old plants, and the AtACTIN2 (AT3G18780) gene was used as a control for equal loading. (B) The survival rates of WT and transgenic A. thaliana plants under HS. The detection of chlorophyll fluorescence (C), soluble protein content (D) and proline content (E) in transgenic and WT plants. The A. thaliana seedlings were cultivated for four weeks under normal temperature (22 °C; Control) before HS (45 °C for 4 h), and the rosette leaves were harvested for the experiments. Data represent the means ± standard deviations of three replicates. Significant differences between WT and transgenic plants are indicated by different letters (P < 0.05). WT: wild-type; OE-8, OE-18, OE-21, OE-28, OE-29, and OE-30: six transgenic A. thaliana lines.