Figure 2.

Effect of Rottlerin treatment on T. gondii proliferation. BeWo cells were infected with T. gondii (host cell: tachyzoites, 1:5) for 3 hours then treated with increasing concentrations of Rottlerin (0.5 µM–5 µM). T. gondii proliferation was evaluated after 24 and 48 hours by a colorimetric microtiter assay using β-galactosidase-expressing tachyzoites. Data were expressed as mean ± SEM of the number tachyzoites calculated in relation to a reference curve and are representative of three independent experiments performed in triplicate. *p < 0.0001: Rottlerin -treated vs control-vehicle cultures at 24 hours; ^#p < 0.01: Rottlerin -treated vs control-vehicle cultures at 48 hours; ^§p < 0.05: Rottlerin-treated cultures at 24 hours vs 48 hours.