Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 25;7:1137. doi: 10.1038/s41598-017-01291-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The reduction of estrogen levels contributes to abnormal fat accumulation and insulin resistance in SERT ^−/− female mice. (a) Representative estrous cycle stage profiles of WT and SERT ^−/− female mice. (b) Plasma 17β-estradiol levels of WT and SERT ^−/− female mice at 3-month age (n = 6 per group). (c) Cyp19a1 mRNA expression in ovary was quantified by RT-qPCR, normalized to GAPDH, and expressed relative to the control group (n = 5–6 per group). (d) Cyp19a1 protein levels in ovary were quantified by western blotting (n = 4 per group). Uncut blots are included in the Supplementary information. (e) Body weight of WT mice and SERT ^−/− female mice treated with placebo or 17β-estradiol for 3 weeks (n = 5–6 per group). (f and g) Representative fat tissues and weight at time of sacrifice (n = 5–6 per group). (h and i) GTT and ITT were performed on SERT ^−/− female mice treated with placebo or 17β-estradiol (n = 5–6 per group). The repeated measures ANOVA P value is provided. The corresponding GTT AUC and ITT AUC were calculated. (j) Fed blood glucose levels were measured (n = 5–6 per group). *P < 0.05. Values are reported as mean ± SEM.