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. 2017 Apr 21;7:1017. doi: 10.1038/s41598-017-01243-z

Figure 4.

Figure 4

The loop portion of SL1 and SLC is highly conserved. (a) The nts within the loop of SLC and SL1 are shown along with the changes introduced in eight loop-specific mutants. The numbers above the nt sequences (L5, L10, L15) mark the positions of the corresponding nts in the loop. The nts at both ends, underlined with dots, make up the two neck base pairs adjacent to the loops. Mutated nts are highlighted with solid underlines, deletions are denoted by spaces. (b) The infectivity of a selected set of loop mutants in bombarded lima bean cotyledons (top row), inoculated (middle row) and systemic (bottom row) soybean leaves. (c) RT-PCR detection of the BR1 and BR2G viral RNAs in lima bean cotyledons bombarded with the loop mutants.