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. 2017 Apr 25;7:1141. doi: 10.1038/s41598-017-01100-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of PstSCR1 reduces infection capacity of oomycete pathogens. (a) The infection of N. benthamiana leaf with P. infestans after expressing ΔSP-PstSCR1-GFP and PstSCR1-GFP constructs. Photographs were taken after 8-dpi. (b) P. Infestans lesion sizes were reduced in leaves expressing PstSCR1-GFP in a SP-dependent manner. Leaf patches expressing PstSCR1-GFP showed significantly smaller lesions compared to leaves expressing SP-GFP, whereas patches expressing ΔSP-PstSCR1-GFP showed similar lesion sizes to those expressing EV-GFP, as measured in pixels (by ImageJ tool). Asterisk indicates significant differences by ttest (*P ≤ 0.05). (c) Peronospora hyoscyami f. sp. tabacina spore count was reduced in leaves expressing PstSCR1-GFP in a SP-dependant manner. N. benthamiana leaves were infected with P. tabacina and spores were counted 8-dpi. Leaf patches expressing PstSCR1-GFP showed significantly less spores than patches expressing SP-GFP, whereas patches expressing ΔSP-PstSCR1-GFP showed similar spores to those expressing EV-GFP. Asterisks indicate significant differences by ttest (***P ≤ 0.001).