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. 2017 Apr 25;7:1141. doi: 10.1038/s41598-017-01100-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PstSCR1 accumulates in the plant apoplast. N. benthamiana plants were co-expressed by agro-infiltration using the following constructs: (a) pGWB454/PstSCR1-RFP and pK7FWG2/EV-GFP, (EV: empty vector), as nucleo-cytoplasmic marker; (b) pGWB454/PstSCR1-RFP and pK7FWG2/PstSCR1-GFP; (c) pGWB454/PstSCR1-RFP and pK7FWG2/ΔSP-PstSCR1-GFP. (d) pGWB454/PstSCR1-RFP and pK7/YFP-REM1.3. (e) pGWB554/C14 (Apoplast and vacuole marker⁴⁷) and pK7WGY2/REM1.3 (Plasma membrane marker^58–60). (d) and (e) indicate PstSCR1 expressed with its SP accumulates in apoplastic space but not at the plasma membrane. (f) The intensity plots illustrate relative RFP and YFP fluorescence signals along the line connecting the points; a-b and c-d in overlayed images of (d) and (e), respectively.