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. 2017 Apr 25;7:1141. doi: 10.1038/s41598-017-01100-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Secretion of PstSCR1 is required to induce cell death in N. benthamiana. (a) The schematic view of constructs used in the experiment. (b) PstSCR1 overexpressed the pTRBO vector with a secretion signal causes HR. Shown are representative N. benthamiana leaf patches expressing pTRBO/PstSCR1, pTRBO/PstSCR1-FLAG, pTRBO/ΔSP-PstSCR1-FLAG and pTRBO/SP-GFP, 4-dpai. (c) Cell death quantification of infiltrated leaf regions. Pixel intensities were normalized by subtracting background in non-infiltrated zone. Error bars represent standard deviations.